Journal: Journal of Oncology

Article Title: The Human Positive Cofactor 4 is a Promising Chemotherapeutic Target in Lung Adenocarcinoma

doi: 10.1155/2021/9958483

Figure Lengend Snippet: The impact of PC4 on the malignant phenotypes of lung adenocarcinoma cells. (a), (b) Western blot assay revealed the protein levels of PC4 after LUAD cells were infected with PC4-knockdown or PC4-overexpression lentivirus. (c) The apoptosis of PC4-knockdown or PC4-overexpression LUAD cells was evaluated by flow cytometry. (d) The growth of PC4-knockdown or PC4-overexpression LUAD cells was analyzed via CCK-8 experiments. (e) The migration of LUAD cells following PC4 knockdown or overexpression was detected via Transwell assay. ∗ p < 0.05.

Article Snippet: The CCK-8 test was accomplished using the Cell Counting Kit-8 (CCK-8) (Bioss, China) as we previously described [ ].

Techniques: Western Blot, Infection, Over Expression, Flow Cytometry, CCK-8 Assay, Migration, Transwell Assay

Journal: Journal of Oncology

Article Title: The Human Positive Cofactor 4 is a Promising Chemotherapeutic Target in Lung Adenocarcinoma

doi: 10.1155/2021/9958483

Figure Lengend Snippet: PC4 decreases cisplatin's cytotoxic effects on lung adenocarcinoma cells in vitro . (a) PC4-knockdown or PC4-overexpression LUAD cells underwent 24-hour treatment with 20 μ M cisplatin. The expression of apoptotic proteins was evaluated. (b) PC4-knockdown or PC4-overexpression LUAD cells were administrated with 20 µ M cisplatin for 96 hours. The CCK-8 assay was performed every 24 hours. (c), (d) PC4-knockdown or PC4-overexpression LUAD cells underwent 24-hour treatment with 0, 20, and 30 µ M cisplatin. Cell apoptosis was measured and analyzed by flow cytometry. ∗ p < 0.05.

Article Snippet: The CCK-8 test was accomplished using the Cell Counting Kit-8 (CCK-8) (Bioss, China) as we previously described [ ].

Techniques: In Vitro, Over Expression, Expressing, CCK-8 Assay, Flow Cytometry