Journal: Cancer Cell International

Article Title: PIBF1 (p.R405Q) germline variant identified in cancer susceptibility family impairs protein stability and function

doi: 10.1186/s12935-025-04132-y

Figure Lengend Snippet: PIBF1 (p.R405Q) mutation is a candidate gene for hereditary cancer syndromes. A . The expression of PIBF1 is low in BRCA patients in the UALCAN database. B . The expression level of PIBF1 in the UALCAN database is negatively correlated with the tumor grade. C . The Kaplan-Meier Plotter plots survival curves of PIBF1 and low expression of PIBF1 was correlated with poor prognosis of breast cancer. D , E . Q-PCR and WB detection of PIBF1-WT and PIBF1(p.R405Q) in the MDA-MB-231 and MDA-MB-468. F . CCK8 was performed to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the cell growth of breast cancer cells. G , H . Plate cloning assay to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the proliferation of breast cancer cells. I . Transwell assay to detect the effect of PIBF1-WT and PIBF1(p.R405Q) on the invasion ability of breast cancer cells. J . WB assay to detect the regulatory effect of PIBF1-WT and PIBF1(p.R405Q) on ERK phosphorylation. All data are presented as mean ± SEM ( n = 3 independent cell culture experiments), *, P < 0.05; **, P < 0.01; ***, P < 0.001

Article Snippet: The medium was refreshed every 24 h with a 1:10 dilution of CCK8 reagent (Cat#C0005, TargetMol, USA) and incubated at 37 °C for 3 hours before measuring absorbance at 450 nm using a microplate reader (EnSpire 2300, PerkinElmer, USA).

Techniques: Mutagenesis, Expressing, Cloning, Transwell Assay, Phospho-proteomics, Cell Culture

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Coupling the near-infrared fluorescent dye IR-780 with cabazitaxel makes renal cell carcinoma chemotherapy possible.

doi: 10.1016/j.biopha.2019.109001

Figure Lengend Snippet: Fig. 3. In vitro effect of Caba-780 on RCC cells and normal human embryonic kidney cells. (A) Cytotoxicity assay using the Cell Counting Kit-8 (CCK-8) method. ACHN, 786-O, and HEK293 cells were incubated with different concentrations (0–20 μM) of Caba-780, IR-780, and cabazitaxel. The IC50 values of Caba- 780 for ACHN, 786-O, and HEK293 cells were 11.5 μM and 1.9 μM, 8.7 μM respectively, and the IC50 values of IR-780 for ACHN, 786-O, and HEK293 were 31.2 μM and 10.4 μM, 5.5 μM, respectively. The IC50 of cabazitaxel could not be calculated for each group. Dose-dependent inhibition of cell growth by Caba-780 was found. The inhibitory effect of Caba-780 on tumor growth was stronger than that of other drugs, and its toxicity to normal cells was weaker than that of IR-780. (B) Plate colony-formation assay. IR-780 had little effect on the clonal growth of the two RCC cell lines (ACHN on the left and 786-O on the right), and the cabazitaxel group had some clone formation, while there was almost no colony formation in the Caba-780 group.

Article Snippet: IR-780 was purchased from Heowns Biological Technology Co., Ltd. (Tianjin, China), cabazitaxel was purchased from Dalian Meilun Biological Technology Co., Ltd. (Dalian, China), sulfobromophthalein sodium (BSP) was purchased from Alfa Aesar Chemical Co., Ltd. (Tianjin, China), rifampin (RIF) was purchased from TCI Development Co., Ltd. (Shanghai, China), sincalide (SIN) and estradiol (EST) were from Medchem Express (MCE, USA), MitoSPy Orange CMTMRos, Lyso Tracker Green DND‐26 and 4′,6-diamidino-2-phenyindole (DAPI) were from Cell Signaling Technology (CST, USA), Cell Counting Kit-8 (CCK8) was from Dojindo Molecular Technologies, Inc. (Tokyo, Japan), RPMI-1640, DMEM, and Fetal bovine serum (FBS) were from Gibco (Gaithersburg, MD, USA), crystal violet, trypsin, dimethyl sulfoxide (DMSO) and paraformaldehyde were from Thermo-Fisher Scientific (Waltham, MA, USA).

Techniques: In Vitro, Cytotoxicity Assay, Cell Counting, CCK-8 Assay, Incubation, Inhibition, Colony Assay